| Identification of P2Y11 Receptor Expressed in Human Retinoblastoma Cells. |
|
Yoon Hee Kim, Dae Ran Kim, Ko I Chun, Jong Hyuck Lee, In Deok Kong |
1Shimmian Oculoplastic Center, Seoul, Korea.
2Department of Basic Nursing Science Keimyung University College of Nursing, Daegu, Korea.
3Department of Ophthalmology, Yonsei University Wonju College of Medicine, Wonju, Korea. jhlee@wonju.yonsei.ac.kr
4Department of Physiology and Institute of Basic Medical Science, Wonju, Korea. |
| 사람 망막아세포종 세포에 발현된 P2Y11 수용체의 확인 |
|
김윤희1,김대란2,전고이3,이종혁3,공인덕4 |
|
Shimmian Oculoplastic Center1, Seoul, Korea Department of Basic Nursing Science Keimyung University College of Nursing2, Daegu, Korea Department of Ophthalmology, Yonsei University Wonju College of Medicine3, Wonju, Korea Department of Physiology and Institute of Basic Medical Science4, Wonju, Korea |
|
Correspondence:
Jong Hyuck Lee, M.D. |
|
|
| Abstract |
PURPOSE
The present study aimed to identify the characteristics and physiological function of the P2Y11 receptor, a receptor likely expressed in human retinoblastoma cells. METHODS: We measured possible P2Y11 signaling in WERI-Rb-1 cells using a Ca2+ imaging technique and RT-PCR. RESULTS: 1) 10 micro M ATP elicited a strong but transient increase in Ca2+ in the WERI-Rb-1 cells, and this Ca2+ rise was well maintained after external Ca2+-depletion. 2) ATP-induced Ca2+ response arose entirely through Ca2+ mobilization. 3) P2Y11 agonist (BzATP, 100 micro M) increased Ca2+ by 31.2+/-3.7 % of ATP effect. 4) mRNA for P2Y11 subtype was identified using RT-PCR. CONCLUSIONS: P2Y11 purinergic activation can increase the intracellular calcium level through calcium mobilization in undifferentiated retinoblastoma cells, which may play an important role in cell proliferation, differentiation, and even pathologic processes. |
| Key Words:
Calcium;P2Y11 receptor;Retinoblastoma cell |
|
PDF Links
Full text via DOI
Download Citation
