| Oxidative Damage induced by UV in Cultured Retinal Pigment Epithelial Cells. |
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Hyeong Gon Yu, Kyung Seek Choi, Jang Won Heo, Kwan Chang Kim, Young Joo Kim, Yesun Chung, Young Suk Yu, Hum Chung |
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Department of Ophthalmology, Seoul National University, College of Medicine, Seoul Artificial Eye center, Seoul National University Hospital, Clinical Research Institute, Korea. chungh@snu.ac.kr |
| 배양망막색소상피세포에서 자외선에 의한 산화손상 |
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유형곤 ( Yu Hyeong Gon ) , 최경식 ( Choe Gyeong Sig ) , 허장원 ( Heo Jang Won ) , 김관창 ( Kim Gwan Chang ) , 김영주 ( Kim Yeong Ju ) , 정예선 ( Jeong Ye Seon ) , 유영석 ( Yu Yeong Seog ) , 정흠 ( Jeong Heum ) |
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| Abstract |
PURPOSE
The death of retinal pigment epithelial(RPE) cells plays an important role in the development of age-related macular degeneration. We studied whether ultraviolet could induce apoptotic cell death and the increase of reactive oxygen intermediates (ROI) in the RPE cells. METHODS: Cultured human RPE cells were exposed to doses of between 0 and 80 mJ/cm2 UVB as an attached monolayer. The number of viable cells was assessed by trypan blue assay. The apoptosis was determined by flow cytometry using annexin V and propidium iodide (PI). Intracellular ROI was measured using DCFH-DA. RESULTS: UVB-exposed cells showed a dose-dependent cell death. The number of cells decreased in 20~30% at the dose of 40 mJ/cm2 UVB 24 hours after UVB exposure. The proportion of annexin V positive cells and intracellular ROI increased after UVB exposure. CONCLUSIONS: These results suggest that UV-induced oxidative damage may be one of the important mechanisms of RPE cell death. |
| Key Words:
Apoptosis;Oxidative damage;Retinal pigment epithelium;UV |
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