| Effect of Cryotherapy on Focal Adhesion Kinase in Retinal Pigment Epithelial Cells. |
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Jun Gyo Lee, Jang Won Heo, Hum Chung |
1Seoul Eye Clinic, Seoul, Korea.
2Department of Ophthalmology, Seoul Municipial Boramae Hospital, Seoul, Korea.
3Department of Ophthalmology, Seoul National University, College of Medicine, Seoul Artificial Eye Center, Seoul National University Hospital, Clinical Research Institute, Seoul, Korea. chungh@snu.ac.kr |
| 냉동치료가 망막색소상피세포의 국소유착분자의 발현에 미치는 영향 |
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이준교1,허장원2,정흠3 |
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Seoul Eye Clinic1, Seoul, Korea Department of Ophthalmology, Seoul Municipial Boramae Hospital2, Seoul, Korea Department of Ophthalmology, Seoul National University, College of Medicine Seoul Artificial Eye Center, Seoul National University Hospital, Clinical Research Institute3, Seoul, Korea |
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Correspondence:
Jun-Gyo Lee, M.D.1 |
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| Abstract |
PURPOSE
In the pathogenesis of proliferative vitreoretinopathy (PVR), retinal pigment epithelial (RPE) cells do not undergo apoptosis after separation from the basement membrane. In order to investigate the mechanism by which RPE cells resist apoptosis in PVR, the expression of focal adhesion kinase after cryotherapy was studied. METHODS: After lensectomy and vitrectomy was performed on rabbit eyes, a retinal tear was created. Cryotherapy was applied to the tear site one week after the tear creation. Rabbits were sacrificed at 1, 4 and 7 days after the tear formation and 30 minutes, 1, 4, 7, 9, 21 days after cryotherapy. Eyes were stained with anti-focal adhesion kinase (FAK) antibody. RESULTS: Expression of FAK increased after the tear formation and remained increased until 21days after cryotherapy. CONCLUSIONS: These data show that creation of a retinal tear and cryotherapy increases FAK expression and may contribute to resistance to apoptosis. |
| Key Words:
Cryotherapy;Focal adhesion kinase;Proliferative vitreoretinopathy;Retinal tear |
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