Lipopolysaccharide-induced Production of Interleukin-8 by Cultured Human Keratocyte.

Lee, Sang Joon; Lee, Dong Jun; Hahn, Young Ho

Original Article

Journal of the Korean Ophthalmological Society 2000;41(10):2051-2059.
Published online October 1, 2000.

Lipopolysaccharide-induced Production of Interleukin-8 by Cultured Human Keratocyte.

Sang Joon Lee, Dong Jun Lee, Young Ho Hahn

Department of Ophthalmology, Kosin University Medical College, #34 Amnam-dong, Seo-ku, Pusan, 602-030, Korea.

배양된 인체 각막간질세포에서 Lipopolysaccharide 투여 후 Interleukin - 8 분비

한영호(Young Ho Hahn),이동준(Dong Jun Lee),이상준(Sang Joon Lee)

Abstract

The authors performed an experiment to determine if human corneal keratocytes release IL-8 after stimulation with lipopolysaccharide (LPS). Human corneal keratocytes were isolated from human corneal buttoms and grown independently in vitro. Cultured keratocytes were treated with various concentrations of LPS (0.01, 0.1 1, 10 microgram/ml). At 6 hours, 12 hours, 24 hours, and 48 hours after the stimulation with LPS, culture supernatants were aspirated and frozen. Supernatants were assayed by enzyme-linked immunosorbent assay for IL-8 content. Exposure of corneal keratocytes to LPS induced IL-8 production. Initially, the secretion of IL-8 was detected at 6 hours and increased upto 48 hours. Between 12 and 24 hours, the IL-8 was increased rapidly. At 6 and 12 hours, keratocytes exposed to 10 microgram / ml LPS produced more IL-8 than those exposed to other concentrations of LPS. In this study, the ability of corneal keratocytes to produce IL-8 upto 48 hours suggests that these cells can play important roles in the induction of the inflammatory response in cornea.

Key Words: IL-8;Keratocyte;Lipopolysaccharide