| Effect of Mitomycin C, Dexamethasone, and Cyclosporine A 0.05% on the Proliferation of Human Corneal Keratocytes. |
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Jong Hoon Shin, Soo Jin Kim, Ji Eun Lee, Jong Soo Lee |
1Department of Ophthalmology, Pusan National University College of Medicine, Busan, Korea. jongsool@pusan.ac.kr
2Department of Ophthalmology, Pusan National University Yangsan Hospital, Busan, Korea. |
| 마이토마이신, 덱사메타존, 사이클로스포린 0.05%가 인간 각막실질세포에 미치는 영향 |
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신종훈1⋅김수진1⋅이지은2⋅이종수1 |
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Department of Ophthalmology, Pusan National University College of Medicine1, Busan, Korea Department of Ophthalmology, Pusan National University Yangsan Hospital2, Busan, Korea |
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| Abstract |
PURPOSE
To investigate the biologic effect of mitomycin C, dexamethasone and cyclosporine A 0.05% on cultured human keratocytes in vitro. METHODS: Human corneal keratocytes were exposed to a concentration of mitomycin C (0.05%), dexamethasone (0.05%) and cyclosporine A (0.05%) for a period of 3, 5, and 10 minutes. MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation and the concentration of type I procollagen COOH-terminal peptide (PIP) and laminin were measured. Cell damage was determined by using the lactate dehydrogenase (LDH) assay. Apoptotic response was evaluated utilizing flow cytometric analysis with Annexin V and propiodium iodide. RESULTS: The inhibitory effect of cellular proliferation and cytotoxicity in cultured human keratocytes showed a time-dependent response in all drugs. The production of PIP and laminin showed a time-dependent response in cultured cells. Apoptosis was observed in flow cytometry after being treated with mitomycin C, dexamethasone and cyclosporine A. Cyclosporin A resulted in less apoptosis of keratocytes than mitomycin C and dexamethasone. CONCLUSIONS: The apoptotic response of mitomycin C, dexamethasone and cyclosporine A is associated with the inhibitory effect of human corneal keratocyte proliferation. To decrease corneal opacity, mitomycin C and dexamethasone were more effective than cyclosporine A in the present study. Additionally, a high concentration of cyclosporine A greater than 0.05% is necessary to lower corneal opacity. |
| Key Words:
Cyclosporine;Dexamethasone;Keratocyte;Mitomycin C |
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