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Effects of Hydrogen Sulfide and Nitric Oxide on the Permeability of Cultured Trabecular Meshwork Cells
황화수소가 섬유주단층세포층 투과도 증가에 미치는 영향과 일산화질소의 상호작용에 관한 연구
JKOS 2020 Mar;61(3):267-73
Published online March 15, 2020;  https://doi.org/10.3341/jkos.2020.61.3.267
Copyright © 2020 The Korean Ophthalmological Society.
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Myung Seo Son, MD, Min Ju Baek, MD, Jae Woo Kim, MD, PhD
손명서⋅백민주⋅김재우

Department of Ophthalmology, Daegu Catholic University College of Medicine, Daegu, Korea
대구가톨릭대학교 의과대학 안과학교실
Received April 4, 2019; Revised August 20, 2019; Accepted February 21, 2020.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Purpose: To investigate the effects of hydrogen sulfide (H2S) on the permeability of a cultured human trabecular meshwork cells (HTMC) monolayer and its interaction with nitric oxide (NO).
Methods: After exposing primary cultured HTMCs to 0, 50, 100, and 500 µM sodium hydrogen sulfide (NaHS) for 6 hours, the permeabilities through the HTMC monolayer were measured using a Transwell assay with carboxyfluorescein. The production of NO and eNOS mRNA expression were assessed using the Griess assay and reverse transcription-polymerase chain reaction, respectively. In addition, 0, 1, and 10 µM NaHS and 10 µM sodium nitroprusside (SN) were co-exposed to evaluate the possible synergistic effect of H2S and NO.
Results: Greater than 100 µM NaHS increased the permeability through the HTMC monolayer in a dose-dependent manner (p < 0.05). These increased permeabilities were not accompanied by NO production or eNOS mRNA expression (p > 0.05). When 0, 1, and 10 µM NaHS and 10 µM SN were exposed together, there was no significant change of permeability, NO production, or eNOS mRNA expression (all, p > 0.05).
Conclusions: NaHS at high concentrations increased the permeability of the HTMC monolayer, which was not affected by NO. NaHS at low concentrations did not show a synergistic effect with NO. Thus, H2S at high concentrations may increase trabecular outflow, which may not be associated with NO.
Keywords : Hydrogen sulfide, Nitric oxide, Permeability, Trabecular meshwork

 

March 2020, 61 (3)