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Effects of Hydrogen Sulfide and Nitric Oxide on the Permeability of Cultured Trabecular Meshwork Cells
황화수소가 섬유주단층세포층 투과도 증가에 미치는 영향과 일산화질소의 상호작용에 관한 연구
JKOS 2020 Mar;61(3):267-73
Published online March 15, 2020;
Copyright © 2020 The Korean Ophthalmological Society.
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Myung Seo Son, MD, Min Ju Baek, MD, Jae Woo Kim, MD, PhD

Department of Ophthalmology, Daegu Catholic University College of Medicine, Daegu, Korea
대구가톨릭대학교 의과대학 안과학교실
Received April 4, 2019; Revised August 20, 2019; Accepted February 21, 2020.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Purpose: To investigate the effects of hydrogen sulfide (H2S) on the permeability of a cultured human trabecular meshwork cells (HTMC) monolayer and its interaction with nitric oxide (NO).
Methods: After exposing primary cultured HTMCs to 0, 50, 100, and 500 µM sodium hydrogen sulfide (NaHS) for 6 hours, the permeabilities through the HTMC monolayer were measured using a Transwell assay with carboxyfluorescein. The production of NO and eNOS mRNA expression were assessed using the Griess assay and reverse transcription-polymerase chain reaction, respectively. In addition, 0, 1, and 10 µM NaHS and 10 µM sodium nitroprusside (SN) were co-exposed to evaluate the possible synergistic effect of H2S and NO.
Results: Greater than 100 µM NaHS increased the permeability through the HTMC monolayer in a dose-dependent manner (p < 0.05). These increased permeabilities were not accompanied by NO production or eNOS mRNA expression (p > 0.05). When 0, 1, and 10 µM NaHS and 10 µM SN were exposed together, there was no significant change of permeability, NO production, or eNOS mRNA expression (all, p > 0.05).
Conclusions: NaHS at high concentrations increased the permeability of the HTMC monolayer, which was not affected by NO. NaHS at low concentrations did not show a synergistic effect with NO. Thus, H2S at high concentrations may increase trabecular outflow, which may not be associated with NO.
Keywords : Hydrogen sulfide, Nitric oxide, Permeability, Trabecular meshwork


March 2020, 61 (3)